Evaluation Form - IHC/IF


Please fill out one evaluation form per antibody.

Antibody Evaluation

* = Required

Name: *

Email: *

Phone: *

Antibody Characterization

Antibody Catalog Number: *

Lot# (begins with QC#):

Antibody Target Gene Name:

How would you rate this antibody on a scale from 1-5 (5 = best) and why?

Would you use this antibody in future experiments?

Have you used another antibody which has worked in your application?

Do you believe the information about the reagent on the web site is correct?

If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not?

How did you store the antibody after re-suspension?

Submitted By: (your name and institution, if you'd like to be credited on the images)

Additional Comments (if any)

Please fill out this page if the antibody was validated by Immunohistochemistry/Immunoflourescence.

Validation: IHC/IF

Sample Description (please include species type and cell/tissue type):

Please explain fixation solution/method used (formalin, periodate-lysine-paraformaldehyde, acetone, etc)?

How many different experimental trials were conducted using the antibody sample?

Primary antibody dilution, incubation time and temperature:

Secondary antibody used, dilution, incubation time and temperature:

From your IHC/IF images, briefly explain the colors of each stain and counterstain:

Did you use an antigen retrieval method? If so, please explain?

What controls were used in your experiment?

Please include your detailed tissue preparation and staining procedure/protocol here:


Please attach IHC/IF image
(Clearly label which antibody goes with which image)

Please include image notes:



Thank you for completing Antibody Verify's antibody evaluation.
If you have any questions, please feel free to call us or email us at info@antibodyverify.com