Product Review: ATP7A Antibody (AAS98337C) in mouse endothelial firboblast and human HUVEC using Western blot

Product Page for ATP7A antibody-middle region (AAS98337C)

Researcher:Anonymous
Application: Western blotting
Species+tissue/cell type:Lane1: 40 ug mouse endothelial firboblast lysate, Lane2: 40 ug human HUVEC lysate
Primary antibody dilution: 1:500
Secondary antibody: Anti-rabbit HRP
Secondary antibody dilution: 1:2000

Questionnaire:
How would you rate this antibody on a scale from 1-5 (5=best) and why? 5- for IB
Would you use this antibody in future experiments? YES
Have you used another antibody which has worked in your application? YES
Do you believe the information about the reagent on Antibody Verify’s website is correct? YES
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? YES
How did you store the antibody after re-suspension? Aliquote in different tube and stored in -20 C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): 1)Mouse, Human 2)fibroblast cells, Endothelial cells, 40 ug protein
How many different experimental trials were conducted using the antibody sample? 1
How was this sample prepared? Cells were lysed with 500 ?l of ice-cold lysis buffer, pH 7.4 (50 mM HEPES, 5 mM EDTA, 150 mM NaCl), 1% Triton X-100, protease inhibitors (10 ?g/ml aprotinin, 1 mM phenylmethylsulfonyl fluoride, 10 ?g/ml leupeptin).
Primary antibody dilution and incubation time: 1:500, overnight
Secondary antibody used and dilution and incubation time: Anti-Rabbit, 1:2000, 1 hr
What controls were used in your experiment (positive/negative)? Positive
Please include your detailed WB Procedure/Protocol here: I followed the instruction for IB from Antibody Verify website .
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