Anti-TP53 antibody (AAS70303C)

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  • (Catalog#: AAS70303C)
100ul
$125.00
In Stock
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Target and Product Information

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Description of Target:
TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Gene Symbol:
TP53
Alias Symbols:
LFS1; TRP53; p53
Protein Accession# :
NP_000537
Nucleotide Accession#:
NM_000546
Swissprot Id:
P04637
Host:
Rabbit
Clonality:
Polyclonal
Protein Size (# AA):
393
Molecular Weight:
44kDa
Application:
WB, CHIP
Partner Proteins:
AES, BIRC3, CARM1, CCND1, CHEK1, CHUK, CREBBP, CREBBP, CXCL10, EGR1, EP300, EP300, FOS, IL8, ING4, JUN, NCOA3, NFKB1, NFKB1, NFKBIA, NFKBIA, NFKBIB, NFKBIB, SLC1A2, TERT, TLE1, AATF, ACTL6A, AES, AHR, AKAP8, APBA2, AR, BRCA1, BTK, BTRC, CALM1, CAMK4, CARM1, CDK9, CEBPB, CEBPD, CHEK1, CHUK, COMMD1, C
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml.
Species Reactivity:
Human
Datasheets / Downloads:
AAS70303C
Blocking Peptide:
Peptide #: EAS70303
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Antibody Reviews and Data(3)

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Product Protocols: TP53 antibody tested with Human 293T Cells (AAS70303C)

Antibody Verify is the original manufacturer of this TP53 antibody (AAS70303C)

Click here to view the TP53 antibody Western Blot Protocol

Product Datasheet Link: TP53 antibody (AAS70303C)

WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T

Western Blot image:


Description of Target: TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Questions pertaining to this data can be directed to techsupport@antibodyverify.com

Antibody Verify’s TP53 antibody (AAS70303C) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@antibodyverify.com.

To order by phone call us at (800) 963-3931, fax us at (702) 425-5639 or send an email to info@antibodyverify.com. Antibody Verify manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Antibody Verify’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@antibodyverify.com

Product Review: TP53 Antibody (AAS70303C) in HeLa cells using Western Blot

Product Page for TP53 antibody- N-terminal region (AAS70303C)

Data Provided by Dr. Dasha Alimentkova and Dr. James Kirkland, University of Guelph

'The p53 antibody is working nicely.'

Sample type: HeLa cells
Antibody titration: 1 to 1000
Gel: 4-15% gradient
Secondary: Goat anti-rabbit HRP
Secondary dilution: 1 to 5000


Product Review: TP53 Antibody (AAS70303C) in DU145 cells using Western Blot

Product Page for TP53 antibody N-terminal region (AAS70303C)

Data Provided by: Dr. Andrei Gartel, University of Illinois at Chicago

DU145 cells were lysed in IP lysis buffer (20mM HEPES, 1% Triton X-100, 150mM NaCl, 1mM


EDTA, 1mM EGTA, 100mM NaF, 10mM Na4P2O7, 1mM Na3VO4, 0.2mM PMSF).


Amount of protein per well: 30ug


Primary antibody conditions: 1:2000 in 5% milk/TBST buffer, overnight at 4°C.


Secondary antibody conditions: 1:5000 in 5% milk/TBST buffer, 1 hour at room temperature.


Protocols & Procedures

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Protocol:
Tips Information: